SDMA ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Intended Use

This CD assay is intended for the quantitative determination of symmetric dimethylarginine (SDMA) in human EDTA plasma and serum.

Contents of Kit

1. PLATE: Holder with precoated strips, 12 x 8 wells
2. STD: Standards diluted in reaction buffer, ready to use (0, 0.1, 0.3, 0.6, 1.5, 4.0 μM), 6 x 1 vial
3. CTRL 1 & CTRL 2: Controls diluted in reaction buffer, ready to use, 2 x 1 vial
4. WASHBUF: ELISA wash buffer concentrate 10x, 2 x 100 ml
5. AB: SDMA antibody, lyophilized, 2 x 1 vial
6. CONJ Conjugate (peroxidase-labeled), concentrate, 1 x 120 μl
7. CONJBUF: Conjugate stabilizing buffer, ready to use, 1 x 24 ml
8. DERBUF: Reaction buffer, ready to use, 1 x 15 ml
9. DER: Derivatization reagent, 2 x 50 mg
10. DMSO: Dimethylsulfoxide (DMSO), 1 x 7 ml
11. CODIL: Dilution buffer after derivatization, ready to use, 1 x 28 ml
12. SUB: TMB substrate (Tetramethylbenzidine), ready to use, 1 x 25 ml
13. STOP: ELISA stop solution, ready to use, 1 x 15 ml

Storage

2-8°C

Precision

Detection Range

Based on internal studies with serum samples of apparently healthy persons (n=40) a mean value of 0.47 μmol/l was calculated. The standard deviation was 0.07 μmol/l.
Mean value ± 2 x standard deviation: 0.47 ± 0.14 μmol/l
Normal range: 0.33 - 0.61 μmol/l
We recommend each laboratory to establish its own reference range.

Sensitivity

The zero-standard was measured 20 times. The detection limit was set as B0 - 2 SD and estimated to be 0.05 μmol/l.

General Description

The dosage of most drugs must be adapted in renal insufficiency, making accurate assessment of renal function a prerequisite in clinical medicine. Furthermore, even a modest decline in renal function has been recognized as a cardiovascular risk. In clinical practice serum creatinine is typically used to asses renal function, but this serum creatinine does not increase at modest decline in renal function. Consequently, there is an ongoing search for suitable endogenous markers of renal function.
SDMA is a methylated derivative of L-arginine which is strictly eliminated by renal extraction, thus SDMA plasma level is strongly correlated to renal function. In 18 studies with more than 2136 patients systemic SDMA concentrations correlated highly with inuline clearance, as well as with various clearance estimates combined and serum creatinine. With respect to this, SDMA exhibits properties of a reliable marker of renal dysfunction. Moreover, there are hints that increased SDMA correlates with total sequential organ failure indicating both renal and hepatic failure and with an increased cardiovascular risk.
##Indication##
• Renal failure
• Cardiovascular risk in renal dysfunction
• Hypertension in renal dysfunction

Standard Curve