Rickettsia typhi IgM ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

An indirect enzyme immunoassay for the detection of IgM class antibody against Rickettsia typhi in human serum or plasma. This kit is for research use only.

Contents of Kit

1. 96-microwell EIA Module: 12 × 8-microwell strips coated with outer membrane protein (rOmpB) extracted from Rickettsia typhi and packaged with desiccant, ready to use.
2. IgM Serum Prep, 10 mL: Buffer containing goat anti-human IgG (Fc(γ)-specific) ready to use.
3. Sample Diluent, 2 × 50 mL: PBS buffer containing bovine serum albumin and Tween 20, ready to use.
4. Enzyme Conjugate, 12 mL: Affinity-purified peroxidase-labeled goat anti-human IgM (μchain-specific), ready to use. Protect from light.
5. Positive Control, 120 μL: Blue cap vial contains reactive human serum pre-diluted 1:10.
6. Cutoff Calibrator, 200 μL: Green cap vial contains equivocally reactive human serum pre-diluted 1:10.
7. Negative Control, 120 μL: Red cap vial contains non-reactive human serum pre-diluted 1:10.
8. TMB Substrate, 12 mL: A solution containing H₂O₂ and tetramethylbenzidine (TMB) supplied in an amber bottle. Ready to use. Protect from light.
9. Stop Solution, 12 mL: Diluted sulfuric acid ready to use. Avoid contact with skin.
10. PBS, 1 liter: Add supplied packet to 1 liter purified water to produce PBS Buffer pH 7.2. Mix thoroughly.
11. Tween 20, 2 mL: Solution of 25% Tween 20 and 75% PBS. Add contents to 1.0 liter PBS to prepare Wash Buffer

Storage

Kit components should be stored at 2-8°C. Bring them to room temperature (20-25°C) before opening bottles and plate pouches. Unused antigen strips should be returned to the package with desiccant and tightly resealed