Rickettsia typhi IgG ELISA Kit

Sample

Serum or plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Rickettsia typhi IgG Antibody kit is intended for the detection and semi-quantitation of IgG class human antibody to Rickettsia typhi.

Contents of Kit

1. EIA Microwells (96)
12 x 8-well strips coated with specific membrane proteinand LPS antigen purified from Rickettsia typhi and packaged with desiccant, ready to use.
2. Sample Diluent, 2 × 50 mL
PBS buffer containing bovine serum albumin and Tween.
3. Enzyme Conjugate, 12 mL
Affinity-purified HRP-labeled goat anti-human IgG (heavy chain) provided ready to use in an amber bottle.
4. Positive Control, 120 μL
Blue cap vial contains reactive human serum, bottled at a 1:10 dilution
5. Cutoff Calibrator, 200 μL
Green cap vial contains equivocally reactive human serum, bottled at a 1:10 dilution.
6. Positive Control, 120 μL
Red cap vial contains non-reactive human serum, bottled at a 1:10 dilution.
7. TMB Substrate, 12 mL
A solution containing H₂O₂ and tetramethylbenzidine (TMB) supplied in an amber bottle. Ready to use. Protect from light.
8. Stop Solution, 12 mL
Diluted sulfuric acid ready to use. May be stored at room temperature.
9. PBS, 1 liter
Add supplied packet to 1 liter purified water to produce phosphate-buffered saline at pH 7.2. Mix thoroughly. For Wash Buffer see Reagent Preparation
10. Tween 20, 2 mL
Solution of 25% Tween 20 and 75% PBS. To make Wash Buffer, see Reagent Preparation

Storage

Kit components should be stored at 2-8°C. Bring them to room temperature (20-25°C) before opening bottles and plate pouches.
Unused antigen strips should be returned to the package with desiccant and tightly resealed After initial opening of test strip bag, use microwells within 3 months.

General Description

Rickettsia typhi is found throughout the world. Human infection by this agent takes the form of murine or endemic typhus, transmitted via infected flea feces. The ensuing infection induces a specific antibody response, which may be detected and used as an indirect means of identifying an infected human.

General Notes

Rickettsia typhi is found throughout the world. Human infection by this agent takes the form of murine or endemic typhus, transmitted via infected flea feces. The ensuing infection induces a specific antibody response, which may be detected and used as an indirect means of identifying an infected human.