Sample
serum, plasma, cell culture supernatant and other biological samples
Intended Use
In vitro quantitative determination of OSM concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8x12 (or 8x6 for 48T). Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2 vial (1 vial for 48T). Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100X), 120ul (60ul for 48T). 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100X), 120ul (60ul for 48T). 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml (5ml for 48T). 2-8°C
6. Sample Dilution Buffer, 20ml (10ml for 48T). 2-8°C
7. Antibody Dilution Buffer, 10ml (5ml for 48T). 2-8°C
8. SABC Dilution Buffer, 10ml (5ml for 48T). 2-8°C
9. Stop Solution, 10ml (5ml for 48T). 2-8°C
10. Wash Buffer(25X), 30ml (15ml for 48T). 2-8°C
11. Plate Sealer, 5 pieces (3 pieces for 48T)
12. Product Description, 1 copy
Storage
2-8°C(for sealed box), please do not freeze! See kit label for expiry date
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Intra-assay Precision:
Sample 1: n=20, Mean=32.42 pg/ml, SD=1.52, CV(%)=4.68
Sample 2: n=20, Mean=125.2 pg/ml, SD=6.8, CV(%)=5.43
Sample 3: n=20, Mean=515 pg/ml, SD=24.82, CV(%)=4.82
Inter-assay Precision:
Sample 1: n=20, Mean=31.73 pg/ml, SD=1.97, CV(%)=6.2
Sample 2: n=20, Mean=131.5 pg/ml, SD=8.31, CV(%)=6.32
Sample 3: n=20, Mean=503 pg/ml, SD=31.39, CV(%)=6.24
Detection Range
15.625-1000pg/ml
Citations
Publication ()
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