Phospholipid Assay Kit

Name: Phospholipid Assay Kit
SKU: DFLA26289
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Phospholipid content in serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Reaction Buffer	10 mL x 1	4°C
Enzyme I	Powder x 1	-20°C
Enzyme II	Powder x 1	-20°C
Dye Reagent	Powder x 1	-20°C, keep in dark
Standard	Powder x 1	4°C
Plate Sealer	3
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.2 mmol/L - 20 mmol/L

General Description

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. Phospholipid Microplate Assay Kit provides a simple and direct procedure for measuring phospholipid content in a variety of samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H O specific dye. The optical density of the pink colored 2 2 product at 570 nm is directly proportional to the phospholipid concentration in the sample.

Citations