Intended Use
Enzyme immunoassay for the separate qualitative and quantitative detection of IgA antibodies against phospholipids in human serum.
Contents of Kit
1. 5 x Sample Buffer: 1 x 20 mL, capped white
2. 50 x Wash Buffer: 1 x 20 mL, capped white
3. Negativee Control: 1 x 1.5 mL,capped green
4. Positive Control: 1 x 1.5 mL, capped red
5. Cut-off Calibrator:1 x 1.5 mL, capped blue
6. Calibrators: 6 x 1.5 mL
7. Conjugates: 1 x 15 mL IgA, capped red
8. TMB Substrate: 1 x 15 mL, capped black
9. Stop Solution: 1 x 15 mL, capped white
10. Microtiterplate: 12 x 8 strips with breakway microwells
Storage
Store all reagents and the microplate at 2-8°C/35-46°F, in their original containers. Once prepared, reconstituted solutions are stable for 1 month at 4°C/39°F, at least. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly.
Precision
Intra-Assay Precision: 3.2%-6.3%
Inter-Assay Precision: 1.6%-5.8%
Citations
Publication ()
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