Okadaic Acid (DSP) ELISA Kit

Koelreuteria paniculata Laxm. has long been cultivated as a traditional ornamental tree in the world, and is developed as a source of natural green food. In this work, five new barrigenol-type triterpenoids, compounds 1-5, were isolated from the seeds of Koelreuteria paniculata Laxm. and elucidated by right of several spectroscopic methods. We studied the impacts of compounds 1-5 on learning and memory impairment induced by okadaic acid (OA) in mice. Notably, compounds 2 and S exhibited great effects in the behavioral experiments. Further, the experiments suggested that compounds 2 and 5 may play a considerable role in regulating the level of glycogen synthase kinase-3 beta (GSK-3 beta) and protein phosphatase 2A (PP2A), thereby reducing hyperphosphorylation of tau protein. In addition, the molecular docking studies were performed on compounds 2 and 5 to initially explore possible molecular mechanisms.

Clean water supply systems and infrastructure require periodic testing of water samples to maintain water quality. To enable this, toxicity tests need to be rapid, accurate, portable, and low cost to provide affordable water security especially in developing countries. Qualitative and quantitative measurement of contaminants in water is classically done by standard analytic chemical methods, which are time-consuming, labor-intensive, expensive, and cannot be performed easily outside the laboratory. An alternative to these methods is cell-based sensors, which can detect the presence of a wide range of toxicants by monitoring the cell states. Although electric cell-substrate impedance sensing (ECIS) has been demonstrated to be an effective method for water toxicity sensing, the use of ECIS only is not sensitive enough to detect many toxicants at low concentrations. Merging a sensor platform with multiparametric measurement capabilities could increase the security of detection. Love mode surface acoustic wave (SAW) device is considered as one of the powerful candidates for cell-based toxicity biosensors. In this study, a hybrid Love mode SAW/ECIS sensor was developed. Theoretical analysis and experiments were performed to demonstrate the capability of two sensing techniques (i.e., SAW and ECIS) for toxicity sensing. (C) 2017 American Institute of Chemical Engineers