Recombinant Hepatitis C Virus Non-structural Protein 5 Genotype-3b, GST-Tagged
Nature
Recombinant
Tag/Conjugate
GST
Purity
HCV NS5 Genotype-3b protein is greater than 95% pure as determined by 10% PAGE (coomassie staining). HCV NS5 Genotype-3b protein was purified by proprietary chromatographic technique.
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10, 000 nucleotides and encodes a single polyprotein of about 3, 000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
Keywords
HCV NS5; Hepatitis C virus non structural protein 5; Hepatitis C virus nonstructural protein 5; Hepatitis C Virus NS5; HCV NS5 Genotype 2a; Hepatitis C Virus NS5 Genotype 2a
Citations
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References
Structure and functions of hepatitis C virus proteins: 15 years after
Since its discovery in 1988, the hepatitis C virus (HCV) has become a hot topic of research by many groups around the world. This globally spread infectious agent is responsible for a large proportion of chronic viral hepatitides. The clue to halting the hepatitis C pandemic may be the detailed understanding of the virus structure, its replication mechanism, and the exact functions of the various proteins. Such understanding could enable the development of new antivirals targeted against hepatitis C virus and possibly an effective vaccine. This review recaps the current knowledge about the HCV genome 15 years after its discovery. The structure and function of particular viral structural (core, E1, E2) and nonstructural (NS2, NS3, NS4, NS5) proteins and noncoding regions known to date are described. With respect to frequent conflicting reports from different research groups, results reproducibly demonstrated by independent investigators are emphasized. Owing to many obstacles and limitations inherent in doing research on this noteworthy virus, the current knowledge is incomplete and the answers to many important questions are to be expected in the future.
MOLECULAR-CLONING OF THE IMMUNODOMINANT REGIONS OF HEPATITIS-C VIRUS TYPE-III AND TYPE-IV BY IMMUNOSCREENING
We have identified the immunodominant regions of hepatitis C virus (HCV) type III and IV by immunoscreening of a lambda gt11 cDNA library, which was constructed with RNA extracted from pooled sera of patients. In addition to the nucleocapsid protein, nonstructural region 3 (NS3), and NS4 regions, we found that the region at the N terminus of NS5 in type III and IV were also immunoreactive as well as in the prototype and type II, although amino acid homologies were approximately 60 % between the prototype (or HCV type II) and HCV isolates of type III or IV. This result indicated that the region at the N terminus of NS5 may be a candidate for serotyping studies of HCV. This report presents the primary structure of immunodominant portions at the N terminus of NS5 in two HCV subtypes and a preliminary study of the immune responses to these antigens.
COA
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