Recombinant Hepatitis C Virus (HCV) NS5, amino acids 2212-2313, genotype 1 immunodominant region. Contains GST fusion partner, was expressed in E. coli. Immunoreactive with HCV positive sera.
> 95% pure (10% PAGE coomassie staining). S-Sepharose, Ceramic Hydroxyapatite and Affinity Purification
50mM Tris-HCl, 5mM EDTA, pH 8.0
2-8°C short term, -20°C long term
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It"s exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication.
HCV; HCV Non-structural protein 5A; Non-structural protein 5A; Flaviviridae; Hepacivirus; Hepatitis C virus; Hepatitis C Virus NS-5a; HCV NS-5a; NS5a; p56; Hepatitis C Virus NS5a; p68