Mouse vitronectin total antigen ELISA Kit

Sample

biological fluids

Species Reactivity

Mouse

Detection Method

Sandwich ELISA

Intended Use

The Mouse vitronectin total antigen ELISA Kit is intended for the quantitative determination of total vitronectin antigen in biological fluids.

Contents of Kit

1. Immunoassay plate: 1-96 well immulon plate (8 x 12 strips removable wells)*, blocked, and dried
2. 10X Wash Buffer: 1 bottle of 50 mL wash; bring to 1 x using DI water
3. Mouse vitronectin Standard: 1 vial lyophilized Standard
4. Anti-mouse vitronectin primary antibody: 1 vial of lyophilized biotinylated polyclonal anti-mouse vitronectin antibody
5. Avidin peroxidase secondary reagent: 1 vial of concentrated HRP labeled avidin
6. TMB substrate solution: 1 bottle of 10 mL solution

Storage

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -70°C for later use. DO NOT
freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

Detection Range

The assay measures total vitronectin in the 5ng/mL-1000ng/mL range.