Detection and quantification of Malondialdehyde (MDA) content in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
Component
Quantity
Storage
96-Well Microplate
1
Assay Buffer
30 mL x 4
4°C
Reaction Buffer I
10 mL x 1
4°C
Reaction Buffer II
1 mL x 1
4°C
Dye Reagent A
Powder x 1
4°C
Dye Reagent B
5 mL x 1
4°C
Standard ( mmol/L)
1
1 mL x 1
4°C
Plate Sealer
3
Instruction Manual
1
Storage
2 - 8°C
Detection Range
0.01 mmol/L - 1 mmol/L
General Description
Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. MDA Microplate Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be measured at a colorimetric readout at 532 nm.
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