Detection and quantification of Low-Density Lipoprotein/Very-Low-Density Lipoprotein (LDL/VLDL) content in serum, plasma, and other biological samples.
Contents of Kit
Component
Quantity
Storage
96-Well Microplate
1
Assay Buffer
30 mL x 4
4°C
Precipitation Buffer
10 mL x 1
4°C
Diluent
30 mL x 1
4°C
Enzyme
Powder x 1
-20°C, keep in dark
Dye Reagent
Powder x 1
4°C, keep in dark
Standard (4mmol/L)
1 mL x 1
4°C
Instruction Manual
1
Storage
2 - 8°C
Detection Range
0.04 mmol/L - 4 mmol/L
General Description
Lipoproteins transport most plasma lipids including cholesterol and triglycerides. High-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL) are the lipoproteins responsible for most of the cholesterol transport in the blood. High LDL levels and low HDL levels are strongly associated with increased risk of adverse cardiovascular events. In this kit, serum HDL and LDL/VLDL are first separated and then the cholesterol concentration of each is determined by a coupled enzyme assay. The products can be measured at a colorimetric readout at 550 nm.
Citations
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