Human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
C2C7
Cell Line Description
Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to an epitope in amino acids 3214 through 3506 on human apolipoprotein B. The antibody blocks binding of LDL to LDL receptors. It does not bind apolipoprotein B48.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
Have you cited CSC-H1253 in a publication? Let us know and earn a reward for your research.
My Review for Mouse Anti-Human LDL Hybridoma [C2C7]
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
