Detection Method
Indirect ELISA
Intended Use
LC1-Ab ELISA is a solid phase enzyme immunoassay employing human recombinant formiminotransferase-cyclodeaminase (cytosolic liver antigen) for the quantitative detection of anti-liver cytosol type 1 autoantibodies (anti-LC-1) in human serum.
Contents of Kit
1. 5X Sample Buffer 1 vial, 20 mL - 5X concentrated
2. 50X Wash Buffer 1 vial, 20 mL - 50X concentrated
1. Negative Control 1 vial, 1.5 mL
2. Positive Control 1 vial, 1.5 mL
3. Calibrators 6 vials, 1.5 mL each 0, 3, 10, 30, 100, 300 U/ml
4. Conjugate 1 vial, 15 mL IgG
5. TMB Substrate 1 vial, 15 mL
6. Stop Solution 1 vial, 15 mL
7. Microtiterplate 12 x 8 well strips
Storage
Store all reagents and the microplate at 2-8°C, in their original containers. Once prepared, reconstituted solutions are stable for 1 month at 4°C, at least. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly. For more detailed information, please download the following document on our website.
Detection Range
0-300 U/mL
Citations
Publication ()
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