Greater than 90.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Format
Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphat buffer pH-7.
Preservative
None
Storage
2-8°C short term, -20°C long term
Introduction
Beta-lactamases are enzymes produced by some bacteria and are responsible for their resistance to beta-lactam antibiotics like penicillins, cephamycins, and carbapenems (ertapenem) (Carbapenems are relatively resistant to beta-lactamase). These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.
Keywords
Cephalosporinase; Penicillinase; Beta-lactamase; Saccharomyces cerevisiae; S. cerevisiae; Saccharomyces; Beta-lactamases; β-lactamase
Citations
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Q: Can you confirm whether the enzyme is bioactive?
A: Yes, One unit will hydrolyze 1.0 μmole of benzyl penicillin at pH 7.0 at 25 °C, in presence of EDTA.
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