Human THPO ELISA Kit

Sample

serum, plasma, cell culture supernatants, urine

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Human THPO ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of human TPO in serum, plasma, cell culture supernatants, and urine.

Contents of Kit

1. TPO Microplate coated with anti-human TPO, 96 wells (12 strips x 8 wells)
2. Wash Buffer Concentrate (20X), 25 mL
3. Recombinant human TPO Standards, 2 vials
4. Assay Diluent A for Standard/Sample (serum/plasma) diluent, 30 mL
5. Assay Diluent B (5X) for Standard/Sample (cell culture medium/urine) diluent, 15 mL
6. Detection Antibody-Biotinylated anti-human TPO, 2 vials (each vial is enough to assay half microplate)
7. Streptavidin-HRP Concentrate (20, 000x), 8 μL
8. TMB One-Step Substrate Reagent (TMB in buffered solution), 12 mL
9. Stop Solution (2 M sulfuric acid), 8 mL

Storage

Stable for up to 6 months from date of shipment at 2-4°C. Store reconstituted standard (recombinant protein) at -80°C. Opened Microplate Wells and reagents are stable for 1 month at 2-4°C. Return unused wells to the pouch containing desiccant pack and reseal along the entire edge.
Note: The whole kit is stable for 1 year when stored at -20°C. Avoid repeated freeze-thaw cycles.

Sensitivity

25 pg/mL