Intended Use
The Human T3 (Total) ELISA Kit is intended for the quantitative determination of triiodothyronine(T3) concentration in human serum.
Contents of Kit
1. Goat Anti-Mouse IgG Coated Microtiter Wells, 96 wells. Ready to use. 1
2. Enzyme Conjugate Concentrate (11×). Concentrated. 1.3 mL
3. Enzyme Conjugate Diluent. Ready to use. 13 mL
4. T3 Reference Standard, 0, 0.75, 1.5, 3.0, 6.0 and 10.0ng/ mL. Ready to use. 1 set, 1.0 mL each
5. Antibody Reagent. Ready to use. 7 mL
6. TMB Reagent (One-Step). Ready to use. 11 mL
7. Stop Solution(1N HCl). Ready to use. 11 mL
Storage
Unopened test kitsshould be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above.
Precision
Intra-Assay: 5.63%
Inter-Assay: 4.96%
Detection Range
0 to 10 ng/mL
Sensitivity
The minimum detectable concentration of T3 by this assay is estimated to be 0.2 ng/ml.
General Description
The thyroid gland exerts powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance, as well as on the maintenance of metabolic activity and the development of the skeletal and organ system. Thehormonesthyroxine(T4) and 3,5,3'triiodothyronine(T3) circulate in the blood stream, mostly bound to the plasma protein, thyroxinebindingglobulin(TBG). The concentration of T3 is much less than that of T4, but its metabolic potency is much greater. T3 determination is an important factor in the diagnosis of thyroid disease. Its measurement has uncovered a variant of hyperthyroidism in thyrotoxic patients with elevated T3 levels and normal T4 levels. An increase in T3 without an increase in T4 is frequently a forerunner of recurrent thyrotoxicosis in previously treated patients. In other patients, euthyroidism is attributable to normal T3, although their T4 values are subnormal. T3 determination is also useful in monitoring both patients under treatment for hyperthyroidism and patients who have discontinued anti-thyroid drug therapy. It is especially valuable in distinguishing between euthyroid and hyperthyroid subjects. In women, T3 levels are elevated during pregnancy, during estrogen treatment, and contraceptive hormone therapy.When T3 levelsparallel TBG increases in a manner analogous to T4 levels, these changes are not a reflection of altered thyroid status.
Standard Curve
Results of a typical standard run with optical density readings at 450 nm shown on the Y-axis against Total T3 concentrations shown in the X-axis. This standard curve is for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain his or her own data and standard curve.
Citations
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