Human/Mouse/Rat AZGP1 ELISA Kit

Sample

cell lysates, culture supernatant, plasma, serum

Species Reactivity

Human; Mouse; Rat

Detection Method

Competitive ELISA

Intended Use

The Human/Mouse/Rat AZGP1 ELISA Kit is an in quantitative assay for detecting ZAG peptide based on the principle of Competitive Enzyme Immunoassay.

Contents of Kit

1. ZAG Microplate
2. Wash Buffer Concentrate (20X) .
3. Standard ZAG Peptide
4. Anti-ZAG polyclonal antibody
5. Assay Diluent A
6. Assay Diluent B
7. Biotinylated ZAG peptide,
8. HRP-Streptavidin concentrate
9. Positive control
10. TMB One-Step Substrate Reagent
11. Stop Solution

Storage

Standard, Biotinylated ZAG peptide, and Positive Control should be stored at -20°C or -80°C (recommended at -80°C) after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at -20°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. If stored in this manner, warranties this kit for 6 months from the date of shipment.

Precision

Intra-assay: CV<10%
Inter-assay: CV<15%

Detection Range

0.1-1000 pg/mL

Sensitivity

21 pg/mL