IL13RA2 (Human) ELISA Kit

Sample

Cell Culture Supernatant, Plasma, Serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

This ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 13 Rα2 concentrations in cell culture supernatant. This kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in diagnostic or therapeutic procedures. FOR LABORATORY RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Contents of Kit

1. IL-13 Rα2 Microtiter Plate: Pre-coated with anti-human IL-13 Rα2 monoclonal antibody. 96 wells
2. Biotin Conjugate: Anti-IL-13 Rα2 polyclonal antibody conjugated to Biotin. 6 mL
3. Avidin Conjugate: Avidin conjugate to horseradish peroxidase. 12 mL
4. IL-13 Rα2 STANDARD: Recombinant human IL-13 Rα2 (12.8 ng/vial) in a buffered protein base with Preservative, lyophilized. 2 vials
5. CALIBRATOR DILUENT I: Animal serum with buffer and preservative. For serum/plasma testing. 25 mL
6. CALIBRATOR DILUENT II: Cell culture medium with calf serum and preservative. For cell culture supernatant testing. 25 mL
7. WASH BUFFER (20×): 20-fold concentrated solution of buffered surfactant. 60 mL
8. SUBSTRATE A: Buffered solution with H₂O₂ 10 mL
9. SUBSTRATE B: Buffered solution with TMB. 10 mL
10. STOP SOLUTION: 2N Sulphuric Acid (H₂SO₄). 14 mL(Caution: Caustic Material!)

Storage

All reagents provided are stored at 2-8°C. Refer to the expiration date on the label.

Sensitivity

The sensitivity of human IL-13 Ra as observed by the standard curve generated for both Calibratory Diluent 1 and Calibrator Diluent II is about 25 pg/mL. It is defined as the detected quantity 2SD from the mean OD of 16 replicates of the zero standards.

General Description

IL-13 receptor alpha 2, also known as CD213a2, is a subunit of IL-13 receptor complex. It is encoded by the gene locus Xq13.1-q28 on chromosome X. An important characteristic of this subunit is that it does not have a cytoplasmic domain. Even though, the IL-13 Ra2 binds to IL 13Ra2 with high affinity, the receptor appears not directly involved with a typical IL-13 stimulated signal transduction. Unlike IL-13aR1, the binding of IL-13Ra2 to IL-13 is IL-4R independent and is not associated with Jaks and the phosphorylation and activation of Stats. There are evidences that soluble IL-13Ra2 may serve as decoy receptor to dampen the cell reaction to IL-13 stimulation.
IL-13Rα2 exists both on the cell membrane, and in soluble form. IL-13Rα2 is relatively rich in hemopoietic or hypervascular tissues, while expressed in low level or not expressed in other tissues. The gene was initially cloned from a kidney cell line and then found to be expressed in brain, spleen, liver, thymus, and also in the bone marrow.
The research on IL-13Ra2 has led to the discovery of association of abnormal IL-13Ra2 expression with a number of pathological conditions. The IL-13Ra2 gene polymorphism was found to be involved with susceptibility to Systemic Sclerosis (Brigitte Granel et al. 2006). The overexpression of Il13ra2 gene has been found in brain tumors and ovarian cancer, making IL-13Ra2 one of the candidates as biomarker and target for immunotherapy. In the research by Mitomu Kioi et al in 2006, IL-13 cytotoxin was highly cytotoxic to the IGROV-1 ovarian cancer cell line in vitro, and it mediated significant antitumor activity against a xenografted tumor model. The result suggested that IL-13Ra2 could be a promising target for ovarian cancer therapy. Vaccine therapy of glioma was tested by vaccination using dendritic cells transfected with Il13ra2 mRNA and this experiment induced strong anti-tumor effects in mice (Makoto Saka et al. 2010).

Standard Curve

Results of a typical standard run of an IL-13 Rα2 ELISA are shown below. Any variation in standard diluent, operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. The following examples are for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain its own results.
The following data was obtained for a standard curve using Calibrator Diluent II.