Human Fentanyl ELISA Kit

Sample

Urine, blood, oral fluid

Species Reactivity

Human

Detection Method

cELISA

Intended Use

For the determination of trace quantities of Fentanyl and/or other metabolites in human urine, blood, oral fluid.

Contents of Kit

1. EIA Buffer: 30 mL (ready-to-use). Phosphate buffered saline solution with bovine serum and a preservative. Provided for dilution of samples.
2. Wash Buffer Concentrate (10×): 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
3. K-Blue Substrate: 20 mL (ready-to-use). Stabilized 3,3',5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H₂O₂ ) in a single bottle. It is used to develop the color in the wells after washing. Light sensitive.
4. Drug-Enzyme Conjugate: 14 mL (ready-to-use). Drug-horseradish peroxidase conjugate. Do not dilute.
5. Antibody Coated Plate: A 96 well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is. Do not wash.
6. Acid Stop Solution: 14 mL (ready-to-use). 1N H₂SO₄ used to stop the enzyme reaction.
7. Qualitative QC Positive Control: 750 μL provided (synthetic human urine). Do not dilute.
8. Qualitative QC Negative Control: 750 μL provided (synthetic human urine). Do not dilute.

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C. Store controls frozen if not used within 10 days. Note: Some kits require controls to be stored frozen immediately upon receipt. Reference kit label for details.

Sensitivity

Fentanyl
I-50 in EIA Buffer: 0.33 ng/mL
I-50 in Human Urine: 0.46 ng/mL
The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

Standard Curve