Human Fentanyl Test Kit

Sample

urine, blood, oral fluid

Species Reactivity

Human

Intended Use

The Human Fentanyl Test Kit is intended for the determination of trace quantities of Fentanyl and/or other metabolites in human urine, blood, oral fluid. Creative Diagnostics's Fentanyl ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C. Store controls frozen if not used within 10 days. Note: Some kits require controls to be stored frozen immediately upon receipt. Reference kit label for details.

Sensitivity

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

General Description

Fentanyl is a synthetic narcotic analgesic of high potency and short duration of action. Though 200 times more potent than morphine, Fentanyl has a high safety margin. The drug is available as a citrate salt in an injectable solution containing 50 μg/mL. It is also available as a transdermal patch containing 2.5-10 mg Fentanyl and provides a dose of 25-100 μg/hr for 72 hours for management of chronic pain. While Fentanyl has all the properties of morphine, it is structurally different and therefore cannot be detected by screening tests for morphine and related opiates. Because of the potency of the drug, concentrations encountered in biological fluids are in the sub nanogram range.

Reconstitution And Storage

1. Desiccant bag must remain in foil pouch with unused strips. Keep ziplock pouch sealed when not in use to maintain a dry environment.
2. Use clean pipette tips for the buffer, drug-enzyme conjugate, controls and samples.
3. Before pipetting a reagent, rinse the pipette tip three times with that reagent.
4. When pipetting into the wells, DO NOT allow the pipette tip to touch the inside of the well or any of the reagent already inside the well. This may result in cross contamination.
5. Controls and samples should be assayed in duplicate.
6. Before substrate addition, wipe the outside bottom of the wells with a lint-free wiper to remove dust and fingerprints.
7. Gently mix specimens and reagents before use. Avoid vigorous agitation.