Human Cytochrome C ELISA Kit

Sample

serum, whole blood, cell lysates

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Human Cytochrome C ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human cytochrome c in cell culture lysates, human whole blood or serum.

Contents of Kit

1. 96-wells precoated microtiter plate coated with monoclonal antibody (murine), 1
2. Plate covers, 4
3. Cytochrome c Standard: 10ng/mL, 2 vials
4. Biotin-Conjugate anti cytochrome c monoclonal (murine) antibody, 1 vial
5. Streptavidin-HRP, 1 vial
6. Assay Buffer Concentrate, 1 vial
7. Wash Buffer Concentrate, 1 bottle
8. Lysis buffer, 1 bottle
9. Subsrate Solution, 1 vial
10. Stop Solution (1 M Phosphoric), 1 vial
11. Green Dye, 1 vial
12. Red Dye, 1 vial

Storage

Store kit reagents at 2-8°C. Immediately after use, remaining reagents should be returned to cold storage (2-8°C). Expiry of the kit and reagents is stated on box front labels. The expiry of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.

Precision

Intra-assay coefficient of variation: 6.0%
Table 5.
Inter-assay coefficient of variation: 3.96%
Table 6.

Sensitivity

0.05 ng/mL