Specificity: F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46).
Host: Human
Application: ELISA, Neut
Summary
Specifications
Antibody Isotype
IgG
Clone
F11.2.32
Species Reactivity
HIV-1
Immunogen
F11.2.32 was prepared by generating a hybridoma cell line in which myeloma cells were fused with splenocytes derived from BALB/c mice immunized with HIV-1 PR in the presence of Freund's adjuvant.
Conjugate
Unconjugated
Applications
Application Notes
Suitable for use in ELISA, Neut. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
General Notes
The Hi-Puri™ Human Anti-HIV-1 Protease Monoclonal antibody (clone F11.2.32, product code DMAB-CPDB017)is a monoclonal antibody specifically directed against HIV-1 Protease. This product is validated for use in ELISA, Neut. F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46). DMAB-CPDB017 is purified by affinity purification and supplied as a liquid formulation in PBS. It is available in multiple sizes, including 100 μg, 200 μg, 500 μg, and 1 mg. For long-term storage, the product should be stored at −20°C and repeated freeze–thaw cycles should be avoided. The product is shipped on dry ice. This product is intended for research use only and is not intended for diagnostic use.
Target
Alternative Names
HIV-1 protease; Human immunodeficiency virus protease; HIV-1 PR; HIV PR; HIV protease
Citations
Publication ()
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