Glutamate Dehydrogenase Assay Kit

Name: Glutamate Dehydrogenase Assay Kit
SKU: DFLA26066
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Tissue extracts, Cell lysate

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Glutamate Dehydrogenase (GDH) activity in tissue extracts, cell lysate samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Substrate Diluent	20 mL x 1	4°C
Substrate	Powder x 1	-20°C
Standard	Powder x 1	-20°C
Positive Control	Powder x 1	-20°C
Instruction Manual	1

Storage

2 - 8°C

Detection Range

4 umol/L - 400 umol/L

General Description

Glutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas. GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH. The assay is initiated with the enzymatic catalysis of NH +, α ketoglutaric acid and NADH by GDH. NADH can be measured at a colorimetric readout at 340 nm.

Citations