Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Glutamate Content in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Dye Reagent A | Powder x 1 | 4°C |
| Dye Reagent A Diluent | 7.5 mL x 1 | 4°C |
| Dye Reagent B | 2.5 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.03 mmol/L - 3 mmol/L
General Description
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods and has been used as a flavor enhancer in food industry. Glutamate Microplate Assay Kit is a sensitive assay for determining glutamate concentration in various samples. The glutamate reacts with the dye and can be measured at a colorimetric readout at 570 nm.
Citations
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