Glucose Uptake Assay Kit

Sample

Whole cells

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport.

Contents of Kit

Storage

2 - 8°C

Detection Range

0.03 mmol/L - 3 mmol/L

General Description

Glucose uptake is an important biological tool for studying cell signaling and glucose metabolism. Glucose uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters which transports glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in urine. Glucose Uptake Colorimetric Microplate Assay Kit provides a simple and direct procedure for measuring glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport. In this reaction, as with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells.