Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Glucose Oxidase (GOD) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Dye Reagent | Powder x 1 | 4°C |
| Dye Reagent Diluent | 1 mL x 1 | 4°C |
| Substrate | Powder x 1 | 4°C |
| Enzyme | Powder x 1 | -20°C |
| Standard ( mmol/L) | 3 | 1 mL x 1 | 4°C |
| Positive Control | Powder x 1 | -20°C |
| Instruction Manual | 1 | |
Detection Range
0.03 mmol/L - 3 mmol/L
General Description
Glucose Oxidase (GOD, EC 1.1.3.4) is an enzyme found in insects, fungi, and bacteria that catalyzes the oxidation of D-glucose to D-gluconolactone. GOD is widely used in the food, beverage, chemical, and pharmaceutical industries. The Glucose Oxidase Activity Microplate Assay Kit provides a simple and direct procedure for measuring GOD activity in a variety of biological samples. GOD activity is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose resulting in the production of hydrogen peroxide (H O ) that reacts with a probe. 2 2
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