Sample
Fish, shrimp, eggs, honey, meat (beef, chicken and pork), milk, milk powder , condensed milk and serum
Intended Use
Chloramphenicol (CAP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in fish, shrimp, eggs, honey, meat (beef, chicken and pork), milk, milk powder , condensed milk and serum.
The unique features of the kit are:
1. High recovery (80-115%), rapid (10-40 minutes), and cost-effective extraction methods.
2. High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb) for shrimp, fish and meat samples.
3. High reproducibility.
4. A quick ELISA assay (less than 1 hour regardless of number of samples).
Contents of Kit
Chloramphenicol (CAP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package.
1. CAP Coated Plate; 1×96-well Plate (8 wells × 12 strips); store at 2-8°C
2. CAP Standards:
a. Negative control (white cap tube); 1.0 mL; store at 2-8°C
b. 0.05 ng/mL (yellow cap tube); 1.0 mL; store at 2-8°C
c. 0.15 ng/mL (orange cap tube); 1.0 mL; store at 2-8°C
d. 0.5 ng/mL (pink cap tube); 1.0 mL; store at 2-8°C
e. 1.5 ng/mL (purple cap tube); 1.0 mL; store at 2-8°C
f. 4.5 ng/mL (blue cap tube); 1.0 mL; store at 2-8°C
g. 10 ng/mL (spiking, optional, red cap tube); 1.0 mL; store at 2-8°C
3. Antibody#1; 6 mL; store at 2-8°C
4. HRP-Conjugated Antibody#2; 6 mL; store at 2-8°C
5. 10× Sample Extraction Buffer**; 25 mL; store at 2-8°C
6. 20× Wash Solution; 30 mL; store at 2-8°C
7. Stop Buffer; 12 mL; store at 2-8°C
8. TMB Substrate; 12 mL; store at 2-8°C
Note: If you are not planning to use the kit for over 1 month, store Standard Stock, Antibody#1 and HRP-Conjugated Antibody#2 at -20°C or in a freezer.
Storage
Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
Citations
Publication ()
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Immuno-PCR in the Analysis of Food Contaminants
Radomirović M, Gligorijević N, Rajković A
Applications: ELISA
Reactive species: Human
"Abstract: Food safety is a significant issue of global concern. Consumer safety and government regulations drive the need for the accurate analysis of food contaminants, residues and other chemical constituents of concern. Traditional methods for the detection of food contaminants often present challenges, including lengthy processing times and food matrix interference; they often require expensive equipment, skilled personnel or have limitations in sensitivity or specificity. Developing novel analytical methods that are sensitive, specific, accurate and rapid is therefore crucial for ensuring food safety and the protection of consumers. The immuno-polymerase chain reaction (IPCR) method offers a promising solution in the analysis of food contaminants by combining the specificity of conventional immunological methods with the exponential sensitivity of PCR amplification. This review evaluates the current state of IPCR methods, describes a variety of existing IPCR formats and explores their application in the analysis of food contaminants, including pathogenic bacteria and their toxins, viruses, mycotoxins, allergens, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, phthalic acid esters, pesticides, antibiotics and other food contaminants. Depending on the type of analyte, either sandwich or competitive format IPCR methods are predominantly used. This review also examines limitations of current IPCR methods and explores potential advancements for future implementation in the field of food safety."
Article snippet: Creative Diagnostics. Chloramphenicol ELISA Kit. Available online: https://www.creative-diagnostics.com/Chloramphenicol-EIA-Kit-123404-464.htm (accessed on 20 October 2024). 
Figure 1. Immuno-PCR formats for chloramphenicol of food contaminants.
COA
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