Sample
Serum, Plasma, Cell culture media, Urine, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of beta-N-Acetylglucosaminidase (NAG) Activity in serum, plasma, cell culture, urine, and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 10 mL x 1 | 4°C |
| Substrate | Powder x 1 | -20°C |
| Dye Reagent | 10 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C |
| Positive Control | 50 μL x 1 | -20°C |
| Instruction Manual | 1 | |
Detection Range
3 umol/L - 300 umol/L
General Description
β-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. NAG concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI. beta-N-Acetylglucosaminidase Microplate Assay Kit is a sensitive assay for determining beta-N-Acetylglucosaminidase activity in various samples. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance. The intensity of the product color, measured at 405 nm, is proportional to the NAG activity in the sample.
Citations
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