Animals were immunized with soluble extracellular cytokeratin. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts type 8 and type 18 cytokeratins in immunoblots. The antibody can be used to detect cytokeratin in formalin or B-5 fixed paraffin sections. The antibody primarily reacts with protein antigens in ductal portions of glands and with primary and metastatic adenocarcinomas.
Application Notes
To detect cytokeratin in formalin or B-5 fixed paraffin sections. Radioactively labeled the antibody can also be used to detect soluble extracellular cytokeratin in a quantitative sandwich RIA.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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