Mouse Anti-Bacteria The Lipoprotein H2 of Pseudomonas aeruginosa Hybridoma [NB2-7]

Immunogen

Purified outer membranes of P. aeruginosa PAO1 strain H1O3

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

NS-1

Clone

NB2-7

Cell Line Description

Animals were immunized with purified outer membranes of P. aeruginosa PAO1 strain H1O3. Spleen cells were fused with NS-1 myeloma cells. The antibody reacted with lipoprotein H2 on 50 of the 52 strains of P. aeruginosa that were tested. The antibody also binds to the P. fluorescens group of the Family Pseudomonadaceae and with Azotobacter vinelandii, but not with other gram negative bacteria. Tested and found negative for ectromelia virus (mousepox).

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO