Lipoprotein A (Lp(a)) Human ELISA Kit

Sample

CSF, Milk, Plasma, Serum, Urine

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Human Lipoprotein A ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of Lp(a) in human plasma, serum, milk, urine, and CSF samples.

Contents of Kit

1. Human Lp(a) Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human Lp(a). 2-8°C
2. Sealing Tapes: Each kit contains 3 precut, pressure sensitive sealing tapes that can be cut to fit the format of the individual assay. 2-8°C
3. Human Lp(a) Standard: Human Lp(a) in a buffered protein base (60 ng, lyophilized). 2-8°C
4. Biotinylated Human Lp(a) Antibody (50×): A 50-fold concentrated biotinylated polyclonal antibody against human Lp(a)[120 μl]. -20°C
5. EIA Diluent Concentrate (10×): A 10-fold concentrated buffered protein base (30 ml). 2-8°C
6. Wash Buffer Concentrate (20×): A 20-fold concentrated buffered surfactant (30 ml, 2 bottles). 2-8°C
7. SP Conjugate (100×): A 100-fold concentrate (80 μl). -20°C
8. Chromogen Substrate (1×): A stabilized peroxidase chromogen substrate tetramethylbenzidine (7 ml). 2-8°C
9. Stop Solution (1×): A 0.5 N hydrochloric acid solution to stop the chromogen substrate reaction (11 ml). 2-8°C

Storage

1. Upon arrival, immediately store components of the kit at recommended temperatures up to the expiration date.
2. Store SP Conjugate and Biotinylated Antibody at -20°C.
3. Store Standard, Microplate, Diluent Concentrate (10×), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8°C.
4. Unused microplate wells may be returned to the foil pouch with the desiccant packs and resealed. May be stored for up to 30 days in a vacuum desiccator.

Performance Characteristics

1. The minimum detectable dose of human Lp(a) as calculated by 2SD from the mean of a zero standard was established to be 0.78 ng/ml.
2. Intra-assay precision was determined by testing three plasma samples twenty times in one assay.
3. Inter-assay precision was determined by testing three plasma samples in twenty assays.

General Description

Lipoprotein(a) [Lp(a)] is an atherogenic lipoprotein particle formed by an assembly of LDL particles and Apo(a) bound to Apo-B100 component of LDL. Apo(a), the main constituent of Lp(a), has serine proteinase activity and is capable of autoproteolysis. Apo(a) has 4548 amino acids, variable sizes from 200 – 700 kDa, multiple isoforms, and structural homology with plasminogen. It competes with plasminogen for its binding site, inhibiting tissue-type plasminogen activator 1 and leading to reduced fibrinolysis. The mean Lp(a) protein level ranges from 78 – 175 μg/ml depending on populations and the risk threshold is 300 μg/ml. High levels of Lp(a) in the blood is a risk factor for myocardial infarction (MI), coronary heart disease (CHD), cerebrovascular disease (CVD), atherosclerosis, thrombosis, and stroke.

Standard Curve

The typical data is provided for reference only. Individual laboratory means may vary from the values listed. Variations between laboratories may be caused by technique differences.