Mouse Anti-X. laevis SRSF10 Hybridoma [2I5]

Immunogen

Mass-isolated nuclei from the oocytes of Xenopus laevis

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

FOX-NY

Clone

2I5

Cell Line Description

Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with the family of 6 essential splicing factors, the SR proteins.

Application Notes

To determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in WB, IP, and cytological assays.

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Synonyms

40 kDa SR repressor protein; FUS interacting protein (Serine arginine rich) 1; FUS interacting protein (serine arginine rich) 2; FUS interacting protein (serine/arginine rich) 1; FUS interacting protein 1; FUS interacting protein 2; FUS interacting serine arginine rich protein; FUS interacting serine arginine rich protein 1; FUSIP 1; FUSIP1; FUSIP1 protein; NSSR; Serine arginine repressor protein; SFR; SFRS 13; SFRS13; Splicing factor arginine/serine rich; Splicing factor arginine/serine rich 13; Splicing factor SRp38; SRp 38; SRp38; SRrp 40; SRrp40; TASR 1; TASR 2; TASR; TASR1; TASR2; TLS associated protein TASR 1; TLS associated protein TASR 2; TLS associated protein with Ser Arg repeats; TLS associated protein with SR repeats; TLS associated serine arginine protein 1; TLS associated serine arginine protein 2; TLS associated serine arginine protein; TLS associated SR protein;

Entrez Gene ID