Anti-S. cerevisiae Polyclonal antibody

Calcium alginate beads, inactivated Saccharomyces cerevisiae and inactivated S. cerevisiae immobilized in calcium alginate beads (S. cerevisiae-calcium alginate beads) are examined as potential biosorption materials as regards their capacity to remove Am-241. In this study, initial experiments were carried out to evaluate the effects of pH (2 and 4) and Am-241 initial concentration: 75, 150, and 300 Bq mL(-1). The experiments were conducted in a batch reactor. Higher removal capacity was observed at pH 2 with the use of S. Cerevisiae, whereas pH 4 performed better for the essays with calcium alginate beads and S. Cerevisiae-calcium alginate beads. The pseudo-first-order kinetic model described the kinetics of biosorption. Calcium alginate was the adsorbent of choice to further experiments with synthetic organic liquid waste. A lower removal rate was observed in the organic waste, although calcium alginate beads have also been able to achieve high sorption capacity in less than 4 h. With the organic waste, the highest value of sorption capacity of Am-241 was 4.38 x 10(-7) mmol g(-1) with an initial Am-241 concentration of 2.31 x 10(-8) mmol L-1.

Methylglyoxal (MG) is a highly reactive aldehyde able to form covalent adducts with proteins and nucleic acids, disrupting cellular functions. In this study, we performed a screening of Saccharomyces cerevisiae (S. cerevisiae) strains to find out which genes of cells are responsive to MG, emphasizing genes against oxidative stress and DNA repair. Yeast strains were grown in the YPD-Galactose medium containing MG (0.5 to 12 mM). The tolerance to MG was evaluated by determining cellular growth and cell viability. The toxicity of MG was more pronounced in the strains with deletion in genes engaged with DNA repair checkpoint proteins, namely Rad23 and Rad50. MG also impaired the growth and viability of S. cerevisiae mutant strains Glo1 and Gsh1, both components of the glyoxalase I system. Differently, the strains with deletion in genes encoding for antioxidant enzymes were apparently resistant to MG. In summary, our data indicate that DNA repair and MG detoxification pathways are keys in the control of MG toxicity in S. cerevisiae.