Bacterially produced fusion protein of Shistosoma japonicum glutathione S-transferase and rabbit OSBP
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
IgG-22I0
Cell Line Description
Animals were immunized with a bacterially produced fusion protein of Shistosoma japonicum glutathione S-transferase and rabbit OSBP (the fusion protein was cleaved, and the glutathione S-transferase removed before immunization). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes OSBP in mammalian tissues and cultured cells.
Application Notes
The antibody is studies of localization and distribution of OSBP in cultured cells, as well as for tracking movement of OSBP after cell perturbation.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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