Anti-M13 monoclonal antibody, clone D74-HG4 [HRP]

We present here that pyrene derivative can be arranged in close proximity using M13 virus as framework through click reaction. The observation of excimer emissions of pyrene was a direct evidence for the proper spatial arrangements of chromophores in the architecture, in which both ground-state and excited-state electronic interactions of pyrenes were facilitated. Efficient energy transfer could be induced through the electrostatic interactions between the M13 backbone and the positively charged rhodamine 6G. The investigation of chromophore-modified M13 and its energy transfer process would be beneficial for exploiting the structures of various natural virus and artificially self-assembled virus-like particles. (C) 2017 Elsevier B.V. All rights reserved.

Herein, we demonstrate an engineered phage mediated rmatrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered, phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various Concentrations of streptavidin or polymer, poly(diallyldirnethylammonium)chltiride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured, on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chernical/gerietic engineering and-Used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.