Anti-M13 fd, F1 monoclonal antibody, clone C63-GF3

Background: Umbelliferone, also known as 7-hydroxycoumarin, is a phenolic metabolite found in many familiar plants. Its derivatives have been shown to have various pharmacological and chemo-preventive effects on human health. A uridine diphosphate glycosyltransferase YjiC from Bacillus licheniformis DSM 13, a cytochrome P450BM3 (CYP450 BM3) variant namely mutant 13 (M13) from Bacillus megaterium, and an O-methyltransferase from Streptomyces avermitilis (SaOMT2) were used for modifications of umbelliferone. Results: Three umbelliferone derivatives (esculetin, skimmin, and herniarin) were generated through enzymatic and whole cell catalysis. To improve the efficiencies of biotransformation, different media, incubation time and concentration of substrate were optimized and the production was scaled up using a 3-L fermentor. The maximum yields of esculetin, skimmin, and herniarin were 337.10 mu M (67.62%), 995.43 mu M (99.54%), and 37.13 mu M (37.13%), respectively. The water solubility of esculetin and skimmin were 1.28-folds and 3.98-folds as high as umbelliferone, respectively, whereas herniarin was 1.89-folds less soluble than umbelliferone. Moreover, the antibacterial and anticancer activities of herniarin showed higher than umbelliferone, esculetin and skimmin. Conclusions: This study proves that both native and engineered enzymes could be employed for the production of precious compounds via whole cell biocatalysis. We successfully produced three molecules herniarin, esculetin and skimmin in practical amounts and their antibacterial and anticancer properties were accessed. One of the newly synthesized molecules the present research suggests that the combinatorial biosynthesis of different biosynthetic enzymes could rapidly promote to a novel secondary metabolite.

Moderate Resolution Imaging Spectroradiometer (MODIS) and Visible Infrared Imaging Radiometer Suite (VIIRS) are major instruments for Earth science observations. Nearly 40 MODIS scientific products and a wide range of VIIRS environmental data records are produced using their global observations. The consistency of the MODIS and VIIRS calibrated data is important for the study of Earth science. This article assesses the calibration consistency of the Aqua MODIS and VIIRS thermal emissive band (TEB) data. To remove the impact of the mismatched relative spectral response (RSR) on the comparisons, the simultaneous nadir observation data from the cross-track infrared sounder (CrIS) and the infrared atmospheric sounding interferometer (IASI) are used as references in two different methods to independently verify the consistency. The comparisons of the MODIS and VIIRS TEB calibrated data show that the brightness temperature (BT) differences for comparable bands between MODIS and VIIRS are in general within 0.2 K for BT larger than 230 K. The differences of all comparable MODIS and VIIRS TEBs are consistent over time. MODIS measurements agree better with N20 VIIRS than with Suomi National Polar-orbiting Partnership (S-NPP) VIIRS. The S-NPP VIIRS measurements are higher than N20 VIIRS, within 0.1 K for long-wave infrared (LWIR) bands and 0.3 K for band M13.