Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k. It cross-reacts with all rat I-A like molecules. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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