Mouse Anti-Human GHR Hybridoma [IHI2.42.4]

We investigated the effects of fermented potato protein (FPP), supplied through drinking water, on growth performance of broiler chickens. A total of 288 male broiler chickens were divided into two groups: a control group and an experimental group supplemented with 0.01% FPP in drinking water. The FPP group had a significantly higher BWG and ADG (P<0.01) and significantly improved FCR (P<0.05). The percentage of breast was significantly higher (P<0.05) in fermented potato protein (FPP) group. The FPP group had an increased villus height (P<0.05) in duodenum and jejunum. Expression of IGF-1 gene in the liver was significantly higher in the FPP group than in the control group (P<0.05), whereas GHR gene expression did not differ between the groups at 37 days. This study suggests that FPP supplementation in drinking water improves growth performance of broiler chickens by stimulating IGF-1 gene expression in the liver and improving the intestinal morphology.

Objective: The effect of a common polymorphism in the Growth Hormone (GH) receptor (d3-GHR) gene on growth, metabolism and body composition was examined in short children born small for gestational age (SGA) on GH treatment. Design: In 96 prepubertal, short SGA children treated with high-dose GH (67 mu g/kg/day) in the NESGAS study, insulin sensitivity (IS), insulin secretion and disposition index (DI) were determined during the first year of treatment. Body composition was analysed by DXA. The d3-GHR locus was determined by simple multiplex PCR. Results: At baseline, children in the d3-GHR group (d3/f1 (n = 37), d3/d3 (n = 7)) had significantly lower IS (median (25-75 percentile)) (223.3% (154.4-304.8)) vs. (269.7% (185.1-356.7)) (p = 0.03) and higher concentrations of glucose (mean (SD)) (4.4 mmol/L (0.6) vs. 4.2 mmol/L (0.7)) (p = 0.03), C-peptide (232.1 pmol/L (168.8-304.1) vs. 185.1 pmol/L (137.7-253.9)) (p = 0.04) and insulin (19.2 pmol/L (11.8-32.2)) vs. (13.7 pmol/L (9.3-20.8)) (p = 0.04) compared to children homozygous for the full length allele (fl/fl-GHR (n = 52)). There were no differences in DI or insulin secretion. Postnatal, spontaneous growth was significantly greater in the d3-GHR group compared to the fl/fl-GHR group (p = 0.02). There were no significant differences in growth response, body composition or metabolism after one year of GH therapy. Conclusion: Short SGA children carrying the d3-GHR polymorphism had increased spontaneous growth, lower IS and a compensatory increase in glucose, C-peptide and insulin before GH therapy compared to children homozyg6us for the full-length allele.