Cell membranes from UT-1 (a cell line derived from the CHO K1 cell line)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
B0
Cell Line Description
Animals were immunized with cell membranes from UT-1 (a cell line derived from the CHO K1 cell line). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with HMG-CoA reductase (the rate limiting enzyme in the cholesterol biosynthetic pathway). Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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