Animals were immunized with the SK MEL 23 human melanoma cells line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with GD2 and GD3 gangliosides. It reacts preferentially with the GD2 and is largely unreactive with other gangliosides.
Application Notes
Diagnosis and therapy of human melanoma.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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