Mouse Anti-FcRn Heavy Chain Heterodimers Hybridoma [3H4]

Immunogen

Soluble neonatal Fc receptor (FcRn) in an adjuvant

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

HL-1 Friendly

Clone

3H4

Cell Line Description

Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody recognizes FcRn heavy chain heterodimers. The IG3 (CRL-2434) antibody recognizes two lipid linked forms of FcRn. These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin). It also recognizes the FcRn-DAF construct. The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct. Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434)and 2G3 (CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains. This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG.

Application Notes

Studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG.

Morphology

Lymphoblast

Growth Properties

Mixed: Adherent and Suspension.

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL.Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper.

Storage

Liquid nitrogen vapor phase.