Soluble neonatal Fc receptor (FcRn) in an adjuvant
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
HL-1 Friendly
Clone
2H4
Cell Line Description
The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992.Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody recognizes FcRn heavy chain heterodimers. FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules. The IG3 (CRL-2434) antibody recognizes two lipid linked forms of FcRn. These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin). It also recognizes the FcRn-DAF construct. The 2G3 (CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct. Unlike 4C9 (CRL-2437), the monoclonal antibodies IG3 (CRL-2434)and 2G3 (CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains. This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. [39787]
Application Notes
Studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG.
Morphology
Lymphoblast
Growth Properties
Mixed: Adherent and Suspension.
CultureMethod
Subculturing
Split ratio: 1:5 every 3 - 4 days. Add fresh medium every 2 - 3 days (depending on cell density). Start culture at 2 X 10^5 viable cells/ml. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks.
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