Animals were immunized with Mono S FPLC purified DNA polymerase epsilon. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon. It does not recognize DNA polymerases alpha, beta or delta. The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Application Notes
It can be used in Western WB techniques to detect the presence of DNA polymerase epsilon.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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