Mice were immunized with fraction VIII human DNA polymerase alpha. Spleen cells were fused with NS-1 myeloma cells. The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species. The cells need to be in culture for 6 to 7 days before antibody production is detectable. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Storage
Liquid nitrogen vapor phase.
Citations
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