E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8.653
Clone
79-2-3
Cell Line Description
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits. Spleen cells were fused with P3X63Ag8.653 myeloma cells.The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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