293-27 cell line (human fetal kidney, transfected to express IL-8 receptor type B)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8U.1
Clone
5E2.6.8
Cell Line Description
Animals were immunized with the 293-27 cell line (human fetal kidney, transfected to express IL-8 receptor type B). Spleen cells were fused with P3X63Ag8U.1 myeloma cells. The antibody binds to IL-8R-B with Kd = 1.9 nM. It does not bind to the type A receptor. The epitope recognized by 4D1.5.7 appears to be expressed in the N terminal portion of the molecule.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Target
Synonyms
CXCR2; chemokine (C-X-C motif) receptor 2; IL8RB, interleukin 8 receptor, beta; C-X-C chemokine receptor type 2; CD182; CMKAR2; CXC-R2; CXCR-2; IL-8R B; GRO/MGSA receptor; IL-8 receptor type 2; interleukin 8 receptor B; chemokine (CXC) receptor 2; interleukin 8 receptor, beta; interleukin 8 receptor type 2; interleukin-8 receptor type B; CXCR2 gene for IL8 receptor type B; high affinity interleukin-8 receptor B; IL8R2; IL8RA; IL8RB; CDw128b;
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