GeneralInformation
Immunogen
Subcutaneously with purified, intact Electrophorus electricus acetylcholine receptor
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Cell Line Description
Animals were immunized subcutaneously with purified, intact Electrophorus electricus acetylcholine receptor. Spleen cells were fused with S194 myeloma cells. The antibody binds to the major immunogenic region of the alpha subunits, and cross-reacts well with acetylcholine receptors of a variety of species. Tested and found negative for ectromelia virus (mousepox).
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Target
Synonyms
CHRNA1; cholinergic receptor, nicotinic, alpha 1 (muscle); achra; achrd; chrna; cms2a; fccms; sccms;
Citations
Publication ()
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COA
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