Mouse Anti Chicken Tubulin Hybridoma [7H8]

Diaporthe taiwanensis sp. nov. (Diaporthales, Sordariomycetes, Ascomycota) isolated on living leaves of Ixora chinensis from Taiwan, is characterized using both phenotypic and genetic characters. Phylogenetic reconstructions based on concatenated DNA sequence data of four nuclear genetic markers (ITS, tef1-alpha, beta-tubulin and cal) show that the new taxon is separated from other representative species of Diaporthe with high statistical support. The new taxon differs from its phylogenetically related congeners mainly by having larger alpha conidia and producing beta conidia in culture and further by host and geographical distribution. Artificial inoculation on leaves confirmed the pathogenicity of the novel fungus to Ixora. The present study is the first report of Diaporthe species causing leaf spots and necrosis on Ixora chinensis in Taiwan.

2- or 4-Substituted 3-N-benzyltriazolylmethyl-13 alpha-oestrone derivatives were synthesised via bromination of ring A and subsequent microwave-assisted, Pd-catalysed C(sp(2))-P couplings. The antiproliferative activities of the newly synthesised brominated and phosphonated compounds against a panel of human cancer cell lines (A2780, MCF-7, MDA-MB 231) were investigated by means of MTT assays. The most potent compound, the 3-N-benzyltriazolylmethyl-4-bromo-13 alpha-oestrone derivative exerted substantial selective cell growth-inhibitory activity against A2780 cell line with a submicromolar IC50 value. Computational calculations reveal strong interactions of the 4-bromo derivative with both colchicine and taxoid binding sites of tubulin. Disturbance of tubulin function has been confirmed by photometric polymerisation assay.