Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected). Spleen cells were fused with Sp2/0-Ag14 cells. The antibody does not block recognition of CD1.1 by T cells. The antibody reacts with CD1.1, and cross-reacts with CD1.2
Morphology
Lymphoblast
Growth Properties
Mixed: Adherent and Suspension. The cells are rather Adherent in nature, and grow best if maintained in Suspension culture plates (e.g., Corning Catalog number 430591).
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, every 2-3 days adjust cell density and maintain cultures between 4x10^5-8x10^5 cells/ml.
My Review for Rat Anti-Mouse Cd1d1 Heterohybridoma [20G9]
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
